Section 2 CHARACTERISATION OF THE ROLE OF BIOPHASE DISTRIBUTION
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چکیده
A rapid and sensitive assay for quantification of nalbuphine, butorphanol and morphine in blood (50μl) and brain microdialysate (~40μl) samples was developed. Blood samples were extracted with ethyl acetate. Analysis was performed with high performance liquid chromatography coupled to an electrochemical detector. The mobile phase was a mixture of 0.1M sodium phosphate buffer, methanol and octane-sulfonic acid with ratio and pH depending on compound and matrix. The limits of quantification in blood samples were 25, 50 and 25 ng/ml for nalbuphine, butorphanol and morphine, respectively and 0.5 ng/ml for morphine in microdialysate samples. Based on sample volume, sensitivity and reproducibility, these assays are particularly suitable for pharmacokinetic-pharmacodynamic studies in rodents.
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